PCR-based detection of microsporidia in silkworms using non-conventional RNA polymerase primers
نویسندگان
چکیده
منابع مشابه
MRPrimerV: a database of PCR primers for RNA virus detection
Many infectious diseases are caused by viral infections, and in particular by RNA viruses such as MERS, Ebola and Zika. To understand viral disease, detection and identification of these viruses are essential. Although PCR is widely used for rapid virus identification due to its low cost and high sensitivity and specificity, very few online database resources have compiled PCR primers for RNA v...
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BACKGROUND & OBJECTIVE Microsporidia are obligate intracellular parasites causing infections predominantly in immunocompromised patients. Enterocytozoon bieneusi is the most important microsporidian causing chronic diarrhoea in AIDS patients. The current method used for diagnosing the microsporidia spores is based on light microscopy using stained smears, which do not differentiate spores at sp...
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We read with great interest the article by Mendez et al. (2) published in a recent issue evaluating different PCR primers for cytomegalovirus (CMV) detection. As CMV PCR becomes a frequently used assay in the management of immunocompromised patients, such as transplant recipients and human immunodeficiency virus-infected individuals, an increasing number of laboratories are establishing in-hous...
متن کاملHemoglobin E detection using PCR with confronting two-pair primers.
OBJECTIVES To develop and apply the polymerase chain reaction with confronting two-pair primers (PCR-CTPP) for detection and identification of hemoglobin E (Hb E). MATERIAL AND METHOD Fifty unrelated northern Thais were included in the present study. DNA was extracted from peripheral blood mononuclear cells and targeted to amplify by PCR-CTPP. The amplified product was analyzed and compared w...
متن کاملDetection and identification of gastrointestinal microsporidia using non-invasive techniques.
AIMS To detect enteric microsporidia in faecal specimens from patients with the acquired immunodeficiency syndrome (AIDS), and to identify the spores to species level without using invasive procedures. METHODS Formalised faecal preparations were examined using a modification of the strong trichrome staining method to demonstrate microsporidian spores. Six positive specimens were prepared for ...
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ژورنال
عنوان ژورنال: Bioscience Biotechnology Research Communications
سال: 2017
ISSN: 0974-6455,2321-4007
DOI: 10.21786/bbrc/10.4/10